Background: One of the typical problems of cell savers is the retransfusion of the heparin added to the system. The aim of this study was to determine whether or not heparin, remaining in the prepared sample of retransfusion blood, might be responsible for disturbance in coagulation and increase in blood loss.Methods: Fifty patients undergoing coronary artery bypass grafting surgery (CABG) were randomly divided into two groups: group C (n=25) received cell-saver blood and group H (n=25) received homologous blood. Volumes of the intraoperative autologous and homologous transfusion, activated clotting time (ACT) of the transfused bloods, and ACT and amount of blood loss in the patients were measured intra and postoperatively.Results: There was no statistical difference between the groups in terms of demographics, preoperative characteristics, or operative details. Cell saver was used in 25 cases, and the average volume of blood autotransfused was 504±158 mL. A significant statistical difference was observed in the mean volumes (460±200 vs.80±160 mL; P=0.0001) of perioperative allogeneic blood transfusions between groups H and C. Despite significant further cell-saver blood transfusion (504±158 cc vs.338±123 cc; P=0.001) and a significantly longer ACT of cell-saver blood than homologous blood (959±85 sec vs.478±58 sec; P=0.0001) intraoperatively, there was no significant difference between the two groups in terms of postoperative blood loss (510±270 cc in group H vs.454±150 cc in group C; P=0.362).Conclusion: Utilization of a cell saver was safe, with no increased risk of bleeding despite heparin added to the system.

Zinc has an important role in skin health and its deficiency reduces immunity to evaluate levels of zinc in squamous cell carcinoma (SCC) and basal cell carcinoma (BCC) of skin, we studied 44 BCC and 38 SCC cases together with 33 controls. Blood samples were taken and after separation of serum, levels of zinc, albumin and total protein were measured by atomic absorption albumin kit and reflectometer consecutively. Serum globulin levels were estimated by subtracting albumin from total protein levels. Zinc levels were significantly lower in cancer cases than control (P<0.001). Mean serum albumin of BCC cases was significantly more than controls (P<0.01), but in BCC cases was not significantly different. Significant correlations were seen between levels of serum zinc and albumin of SCC (r=0.507, P<0.01) and BCC(r=0.43, P<0.01) patients and controls(r=0.35,P<0.01). Mean globulin levels in cancer cases were significantly lower than control group (P<0.01).We concluded that serum zinc and globulin levels decrease in BCC and SCC of skin.

Milk thistle (Silybum marianum (L.) Gaertn) belonging to the Asteraceae family and known for its valuable secondary metabolite, silymarin. In order to get the cell suspension culture of Silybum marianum, the combination of 2,4-D and BAP hormones each with five different concentrations (0, 0.1, 1, 2 and 5 mg/L) and 3 different seedling explants (cotyledon, hypocotyl and root) was used to callus formation. High-quality callus was observed in eight different of hormone-explant combinations. Successful cell suspension culture was achieved only by using callus created from cotyledon explants treated with 0.1 mg/L 2,4-D and 5 mg/L BAP. Furthermore, the study examined the effects of 2% and 4% Serendipita indica cell wall extract as an elicitor on silymarin production in cell suspension culture at three different inoculation times (24, 48, and 72 h). After 48 h of inoculation with 2% fungal extract, the highest level of silymarin (199 ppm) was observed, which was significantly different from the control (46 ppm). The silymarin content of cells increased over time through elicitation with 4% fungal elicitor, while no similar pattern was observed with 2% fungal extract. Based on the results, the cell wall of S. indica can significantly enhance the amount of SLM in the cell suspension culture of S. marianum.

Basal cell carcinoma is the, most common skin cancer that has nodular, superficial, micronodular, Morpheaform, pigmented, metatypical and fibroepithelioma of Pinkus subtypes. In this report, we evaluated the efficacy of cryosurgery with liquid nitrogen for basal cell carcinoma in three patients. The response was good and patients were followed regularly. There were no recurrences after 25 months follow-up. This method is fast and appropriate for certain types of basal cell carcinomas.      

In this study a 31 y/o woman who was housewife, came from Mazandaran and lived in Behshahr is presented. Patient's complaints were diarrhea, bloating, abdominal pain and weight loss. Abdominal radiography showed loop dilatation. In primary Para clinic assessments she had Alb: 2.8, Ca: 6.4, P: 2.3 and PTH: 201. Considering malabsorption in early evaluation, upper endoscopy was done. Intestinal folds were disappeared in second and third part of duodenum in endoscopic view and duodenal biopsy was done. Then pathological assessment revealed a large accumulation of intraepithelial lymphocytes and obvious villus atrophy that was accordant to celiac sprue. She was advised to be on gluten free diet. After about 1 month, She was referred because of sever lower extremities edema. Serum albumin was checked that was 2. Enlarged mesenteric lymph nodes were detected in sonography. Considering non- responding treatment, IHC staining of intestinal biopsy was performed with celiac sprue complication suspicion.Therefore the patient was referred to chemotherapy center with the diagnosis of T-cell lymphoma following celiac sprue. She was spent 6 course chemotherapy with CHOP regimen and radiotherapy.